A series of novel chemically-modified amyloses (AMs) or celluloses (CEs) were homogenously bonded onto high-quality porous silica particles in the amylose-immobilized ChiralAM columns or cellulose-immobilized ChiralCE columns via stable covalent bonds. Therefore, both standard and non-standard mobile phases can be used to optimize chiral separations in multi-mode chromatography. The ChiralCD series of chiral columns are new types of chemically-substituted cyclodextrin(CD)-bonded columns. The ChiralCD particles were synthesized by bonding a series of novel functional groups-substituted α-, β-, or γ-cyclodextrins onto surface of high-quality porous silica support by linking the spacer arms at the wider torus rims of the cyclodextrins. The ChiralAMCD, ChiralAMCE, and ChiralCECD columns are firstly created and produced by ChiralTek via immobilizing proprietary complex selectors containing two different chiral recognition moieties. They are the first type of commercial columns immobilizing the innovative complex selectors. Due to the co-operative functioning of the two different types of chiral recognition moieties of the novel complex selectors, the ChiralAMCD, ChiralAMCE, and ChiralCECD columns have exhibited excellent chiral recognition for a wide range of chiral compounds.

1.1. ChiralAMCD Columns

1.6. ChiralCD Columns

ChiralCD columns are a series of new types of chemically-substituted cyclodextrin-bonded silica particles-packed chiral columns. The ChiralCD particles were synthesized by bonding a series of different functional groups-substituted α-, β-, or γ-cyclodextrins onto surface of high-quality porous silica gel particles by linking the spacer arms at the wider torus rim of the cyclodextrins as shown in the following Figure (J). Due to the multiple functional groups, e.g., halogen groups, hydroxyl groups, aromatic rings, and cyclodextrin moiety etc., available in the bonded stationary phases, the ChiralCD columns can be used under multiple modes of mobile phase conditions in UPLC, SFC, and HPLC.

Figure (J). Schematic diagram of the CD-bonded ChiralCD phase.

At present, all the cyclodextrin-based chiral columns from other suppliers are prepared by anchoring cyclodextrin through the more reactive primary hydroxyl groups at the narrow torus rim of cycoldextrins. Since the ChiralCD columns are the first commercial chiral columns by anchoring cyclodextrins through the less reactive secondary hydroxyl at the wider torus rim of the cyclodextrins, the ChiralTek proprietary chiral spacer arms can provide extra unique steric interactions with solutes when entering the cyclodextrin cavities through the wider torus rim. Therefore, excellent separation selectivity can be achieved on the ChiralCD columns for separation a wide range of both chiral and non-chiral compounds. Since the ChiralCD phases from ChiralTek contain higher content of bonded cyclodextrins than other supplier’s cyclodextrin-bonded phases, the ChiralCD columns can exhibit much higher sample capacity. Due to the different chemical structure of the substituted cyclodextrins in ChiralCD phases, the new ChiralCD columns can provide different and generally better separation abilities than other supplier’s cyclodextrin-based columns.

2. Universal HLE Cartridges and Ultra-high Performance UPE cartridges for SPE

The Hydrophilic and Lipophilic Extraction (HLE) cartridges are a new type of universal solid phase extraction (SPE) cartridges manufactured by ChiralTek. The HLE packing materials were prepared by bonding multiple chemical function groups, including Diol, C18 (ODS), vinylpyrrolidone and divinylbenzene (HLB) etc., onto special type of porous composite materials. By using a ChiralTek proprietary packing approach, the HLE packing materials were then packed into kinds of polypropylene tubes with different sizes to produce many models of HLE cartridge products. Since the novel HLE cartridges contain multiple chemical function groups, they can be used under traditional normal and reversed phase elution conditions and modern gradient elution conditions to extract and to fractionate types of hydrophilic and lipophilic compounds from complicated samples. The HLE cartridges can also be used as direct replacement of HLB cartridges due to functioning of vinylpyrrolidone and divinylbenzene groups in the HLE particles.

The Ultra-high Performance Extraction (UPE) cartridges are a novel type of SPE cartridges manufactured by ChiralTek. The UPE particles were prepared through a specially-designed procedure by bonding C18 (ODS) and ChiralTek proprietary functional groups (R) onto high-quality porous spherical silica particles. Due to the unique co-operative functioning of the bonded C18 (ODS) and ChiralTek proprietary functional groups (R), the UPE cartridges exhibit excellent column efficiency, high reproducibility, high elution efficiency, and very high extraction recovery. The specially-designed synthetic procedure allows the UPE particles to contain ultra-high content of ODS functional group. This directly results in ultra-high sample capacity for the novel UPE cartridges containing ultra-small amount of packing materials. Therefore, the UPE cartridges only need ultra-small volume of organic solvents to achieve eluting and concentrating in one step. The novel UPE cartridges are idea choices for trace analysis when working with the PSPE, MFA & f-SPE instruments.

3. Novel Sample Preparation Instruments PSPE, MFA, and f-SPE